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n m syto green  (Keygen Biotech)

 
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    Keygen Biotech n m syto green
    Neutrophil activation and NETs formation mediated by fibrinogen. a) H&E staining of rat subcutaneous scaffolds 1 h postimplantation. b) Immunofluorescence costaining of fibrinogen and MPO. c–e) Serial paraffin sections were subjected to immunofluorescence costaining for: c) MPO and CitH3, d) MPO and NE, e) MPO and CD177. Nuclei were counterstained with Sytox Green. <t>f)</t> <t>dHL‐60</t> cells were seeded onto PBS/hFibrinogen‐coated dishes. Morphological changes were observed by labeling cell membranes with Dil and nuclei with Sytox Green. g) Immunofluorescence costaining for MPO and CitH3 in dHL‐60 after 1 h, with nuclei counterstained with Sytox Green. h) 3D coculture scheme: Fibrinogen‐preadsorbed scaffolds were seeded with dHL‐60 cells for 1 h. i) Cell membranes were labeled with Dil, and the nuclei with <t>Syto</t> Green.
    N M Syto Green, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n+m+syto+green/pmc12904018-324-6-10?v=Keygen+Biotech
    Average 86 stars, based on 1 article reviews
    n m syto green - by Bioz Stars, 2026-07
    86/100 stars

    Images

    1) Product Images from "Elevator‐Like Hollow Channels in Porous Scaffolds Accelerate Vascularized Bone Regeneration via NETs‐Fibrin‐Mediated Macrophage Recruitment"

    Article Title: Elevator‐Like Hollow Channels in Porous Scaffolds Accelerate Vascularized Bone Regeneration via NETs‐Fibrin‐Mediated Macrophage Recruitment

    Journal: Advanced Science

    doi: 10.1002/advs.202515693

    Neutrophil activation and NETs formation mediated by fibrinogen. a) H&E staining of rat subcutaneous scaffolds 1 h postimplantation. b) Immunofluorescence costaining of fibrinogen and MPO. c–e) Serial paraffin sections were subjected to immunofluorescence costaining for: c) MPO and CitH3, d) MPO and NE, e) MPO and CD177. Nuclei were counterstained with Sytox Green. f) dHL‐60 cells were seeded onto PBS/hFibrinogen‐coated dishes. Morphological changes were observed by labeling cell membranes with Dil and nuclei with Sytox Green. g) Immunofluorescence costaining for MPO and CitH3 in dHL‐60 after 1 h, with nuclei counterstained with Sytox Green. h) 3D coculture scheme: Fibrinogen‐preadsorbed scaffolds were seeded with dHL‐60 cells for 1 h. i) Cell membranes were labeled with Dil, and the nuclei with Syto Green.
    Figure Legend Snippet: Neutrophil activation and NETs formation mediated by fibrinogen. a) H&E staining of rat subcutaneous scaffolds 1 h postimplantation. b) Immunofluorescence costaining of fibrinogen and MPO. c–e) Serial paraffin sections were subjected to immunofluorescence costaining for: c) MPO and CitH3, d) MPO and NE, e) MPO and CD177. Nuclei were counterstained with Sytox Green. f) dHL‐60 cells were seeded onto PBS/hFibrinogen‐coated dishes. Morphological changes were observed by labeling cell membranes with Dil and nuclei with Sytox Green. g) Immunofluorescence costaining for MPO and CitH3 in dHL‐60 after 1 h, with nuclei counterstained with Sytox Green. h) 3D coculture scheme: Fibrinogen‐preadsorbed scaffolds were seeded with dHL‐60 cells for 1 h. i) Cell membranes were labeled with Dil, and the nuclei with Syto Green.

    Techniques Used: Activation Assay, Staining, Immunofluorescence, Labeling

    NETs drive macrophage infiltration into channel structures. a) MPO⁺ neutrophils and CD68⁺ macrophages in subcutaneously implanted scaffolds at 4 h, with nuclei counterstained using Sytox Green. b) H&E staining at 8 h postimplantation, along with immunofluorescence costaining of MPO and CD68, with Sytox Green nuclei counterstain. c) Left: Masson's trichrome staining at 24, 48 h; Right: CD68⁺ macrophages in serial sections. d) Gelation assay: A mixture of fibrinogen solution and dHL‐60 cell suspension transitioned from a liquid to a gel state within 3 h. e) Schematic showing the design of the 3D coculture experiment to assess the chemotactic effect of NETs on THP‐1 cells. f) THP‐1 cells (Dil, red) were seeded on the surface of NETs (Syto Green, green)/fibrin gel, and imaged at specified time points. g) Schematic of the Transwell assay for evaluating the chemotactic effect of NETs on THP‐1 cells. h) Migrated THP‐1 cells (crystal violet⁺) at 24 h. i) Quantification and statistical analysis of migrated THP‐1 cells ( n = 6 biological replicates). Data were analyzed using unpaired t ‐tests, with statistical significance defined as **** p < 0.0001.
    Figure Legend Snippet: NETs drive macrophage infiltration into channel structures. a) MPO⁺ neutrophils and CD68⁺ macrophages in subcutaneously implanted scaffolds at 4 h, with nuclei counterstained using Sytox Green. b) H&E staining at 8 h postimplantation, along with immunofluorescence costaining of MPO and CD68, with Sytox Green nuclei counterstain. c) Left: Masson's trichrome staining at 24, 48 h; Right: CD68⁺ macrophages in serial sections. d) Gelation assay: A mixture of fibrinogen solution and dHL‐60 cell suspension transitioned from a liquid to a gel state within 3 h. e) Schematic showing the design of the 3D coculture experiment to assess the chemotactic effect of NETs on THP‐1 cells. f) THP‐1 cells (Dil, red) were seeded on the surface of NETs (Syto Green, green)/fibrin gel, and imaged at specified time points. g) Schematic of the Transwell assay for evaluating the chemotactic effect of NETs on THP‐1 cells. h) Migrated THP‐1 cells (crystal violet⁺) at 24 h. i) Quantification and statistical analysis of migrated THP‐1 cells ( n = 6 biological replicates). Data were analyzed using unpaired t ‐tests, with statistical significance defined as **** p < 0.0001.

    Techniques Used: Staining, Immunofluorescence, Suspension, Transwell Assay



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    n m syto green  (Keygen Biotech)
    86
    Keygen Biotech n m syto green
    Neutrophil activation and NETs formation mediated by fibrinogen. a) H&E staining of rat subcutaneous scaffolds 1 h postimplantation. b) Immunofluorescence costaining of fibrinogen and MPO. c–e) Serial paraffin sections were subjected to immunofluorescence costaining for: c) MPO and CitH3, d) MPO and NE, e) MPO and CD177. Nuclei were counterstained with Sytox Green. <t>f)</t> <t>dHL‐60</t> cells were seeded onto PBS/hFibrinogen‐coated dishes. Morphological changes were observed by labeling cell membranes with Dil and nuclei with Sytox Green. g) Immunofluorescence costaining for MPO and CitH3 in dHL‐60 after 1 h, with nuclei counterstained with Sytox Green. h) 3D coculture scheme: Fibrinogen‐preadsorbed scaffolds were seeded with dHL‐60 cells for 1 h. i) Cell membranes were labeled with Dil, and the nuclei with <t>Syto</t> Green.
    N M Syto Green, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/n+m+syto+green/pmc12904018-324-6-10?v=Keygen+Biotech
    Average 86 stars, based on 1 article reviews
    n m syto green - by Bioz Stars, 2026-07
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Neutrophil activation and NETs formation mediated by fibrinogen. a) H&E staining of rat subcutaneous scaffolds 1 h postimplantation. b) Immunofluorescence costaining of fibrinogen and MPO. c–e) Serial paraffin sections were subjected to immunofluorescence costaining for: c) MPO and CitH3, d) MPO and NE, e) MPO and CD177. Nuclei were counterstained with Sytox Green. f) dHL‐60 cells were seeded onto PBS/hFibrinogen‐coated dishes. Morphological changes were observed by labeling cell membranes with Dil and nuclei with Sytox Green. g) Immunofluorescence costaining for MPO and CitH3 in dHL‐60 after 1 h, with nuclei counterstained with Sytox Green. h) 3D coculture scheme: Fibrinogen‐preadsorbed scaffolds were seeded with dHL‐60 cells for 1 h. i) Cell membranes were labeled with Dil, and the nuclei with Syto Green.

    Journal: Advanced Science

    Article Title: Elevator‐Like Hollow Channels in Porous Scaffolds Accelerate Vascularized Bone Regeneration via NETs‐Fibrin‐Mediated Macrophage Recruitment

    doi: 10.1002/advs.202515693

    Figure Lengend Snippet: Neutrophil activation and NETs formation mediated by fibrinogen. a) H&E staining of rat subcutaneous scaffolds 1 h postimplantation. b) Immunofluorescence costaining of fibrinogen and MPO. c–e) Serial paraffin sections were subjected to immunofluorescence costaining for: c) MPO and CitH3, d) MPO and NE, e) MPO and CD177. Nuclei were counterstained with Sytox Green. f) dHL‐60 cells were seeded onto PBS/hFibrinogen‐coated dishes. Morphological changes were observed by labeling cell membranes with Dil and nuclei with Sytox Green. g) Immunofluorescence costaining for MPO and CitH3 in dHL‐60 after 1 h, with nuclei counterstained with Sytox Green. h) 3D coculture scheme: Fibrinogen‐preadsorbed scaffolds were seeded with dHL‐60 cells for 1 h. i) Cell membranes were labeled with Dil, and the nuclei with Syto Green.

    Article Snippet: dHL‐60 nuclei were stained with 20 n m Syto Green (KeyGEN Biotech, Nanjing, China) for 20 min.

    Techniques: Activation Assay, Staining, Immunofluorescence, Labeling

    NETs drive macrophage infiltration into channel structures. a) MPO⁺ neutrophils and CD68⁺ macrophages in subcutaneously implanted scaffolds at 4 h, with nuclei counterstained using Sytox Green. b) H&E staining at 8 h postimplantation, along with immunofluorescence costaining of MPO and CD68, with Sytox Green nuclei counterstain. c) Left: Masson's trichrome staining at 24, 48 h; Right: CD68⁺ macrophages in serial sections. d) Gelation assay: A mixture of fibrinogen solution and dHL‐60 cell suspension transitioned from a liquid to a gel state within 3 h. e) Schematic showing the design of the 3D coculture experiment to assess the chemotactic effect of NETs on THP‐1 cells. f) THP‐1 cells (Dil, red) were seeded on the surface of NETs (Syto Green, green)/fibrin gel, and imaged at specified time points. g) Schematic of the Transwell assay for evaluating the chemotactic effect of NETs on THP‐1 cells. h) Migrated THP‐1 cells (crystal violet⁺) at 24 h. i) Quantification and statistical analysis of migrated THP‐1 cells ( n = 6 biological replicates). Data were analyzed using unpaired t ‐tests, with statistical significance defined as **** p < 0.0001.

    Journal: Advanced Science

    Article Title: Elevator‐Like Hollow Channels in Porous Scaffolds Accelerate Vascularized Bone Regeneration via NETs‐Fibrin‐Mediated Macrophage Recruitment

    doi: 10.1002/advs.202515693

    Figure Lengend Snippet: NETs drive macrophage infiltration into channel structures. a) MPO⁺ neutrophils and CD68⁺ macrophages in subcutaneously implanted scaffolds at 4 h, with nuclei counterstained using Sytox Green. b) H&E staining at 8 h postimplantation, along with immunofluorescence costaining of MPO and CD68, with Sytox Green nuclei counterstain. c) Left: Masson's trichrome staining at 24, 48 h; Right: CD68⁺ macrophages in serial sections. d) Gelation assay: A mixture of fibrinogen solution and dHL‐60 cell suspension transitioned from a liquid to a gel state within 3 h. e) Schematic showing the design of the 3D coculture experiment to assess the chemotactic effect of NETs on THP‐1 cells. f) THP‐1 cells (Dil, red) were seeded on the surface of NETs (Syto Green, green)/fibrin gel, and imaged at specified time points. g) Schematic of the Transwell assay for evaluating the chemotactic effect of NETs on THP‐1 cells. h) Migrated THP‐1 cells (crystal violet⁺) at 24 h. i) Quantification and statistical analysis of migrated THP‐1 cells ( n = 6 biological replicates). Data were analyzed using unpaired t ‐tests, with statistical significance defined as **** p < 0.0001.

    Article Snippet: dHL‐60 nuclei were stained with 20 n m Syto Green (KeyGEN Biotech, Nanjing, China) for 20 min.

    Techniques: Staining, Immunofluorescence, Suspension, Transwell Assay